De-erythrocytosis PCR Kit
Product Description:
For non-fresh blood
For non-fresh blood
· Universal—One master mix for different sample types, one annealing temperature for different primer sets
· Convenient—Buffer formulated for primer annealing at 60°C, helping simplify PCR optimization
· Fast—No DNA purification, no cross contamination
· Efficient— High inhibitor tolerance; GC enhancer included for amplification of GC-rich sequences
· Flexible—Optional lysis-and-storage protocol for long-term storage and further testing
· Fewer pipetting—Master mix format with direct gel-loading dye
AJdicrect De-erythrocytosis PCR Kit
AJdicrect De-erythrocytosis PCR Kit
AJ-4102-50
AJ-4102-200
50 T/package
200 T/package
· Take 50 μL non-fresh blood in 1.5mL centrifuge tube, add 1,000 μL Extraction Buffer and mix by vortex. Centrifuge at 13,000 rpm for 1 minute. Save the pellet and discard the supernatant.
· Add 60 μL Lysis Buffer to the centrifuge tube containing pellet, vortex ensures that the pellet was completely suspended.
· Incubate at room temperature for 1 minute, then add 60 μL Neutralization Solution and vortex, resuspended the solution can be used as the DNA template for PCR.