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SmartDx Personalized Medication Guide

Intended use:

Single nucleotide polymorphisms (SNPs) are the commonest genetic variant in the human genome and have been associated with inter-individual differences in drug response. Finding the causative SNPs underlying variations in drug response has been a cornerstone of personalized medicine. AJcorre SNP assays presented a pragmatic platform toward simple and fast SNP genotyping in research laboratories, and a potential tool to improve the widespread application of personalized medicine

Product Features Experiment Data Personalized Medication Testing Reference Chart AJ-Q9600 Multi-Channel Fluorescence Quantitative Analyzer Application scenario

Product Features

The assay is based on TaqMan technology employing specific primers and fluorescence probes, generating a dual-fluorescence signals. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencer dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye which is monitored real-time by PCR detection system. The PCR cycle at which an increase in the fluorescent signal is detected initially (Ct) is proportional to the amount of the specific PCR product.

Examples of results

 

FAM signalFAM/VIC信号VIC信号标识Wild Type
FAM/VIC signalFAM信号VIC信号标识Heterozygous Type
VIC signalFAM信号FAM/VIC信号标识Mutagenic Type
No signalFA M信号VIC信号FAM/VIC  NTC

Personalized Medication Testing Reference Chart

Medication Associated SNP
Platinum

XRCC1

GSTP1(I105V)

ERCC1(C118T）
Anthracyclines

ABCB1(C3435T)

GSTP1(I105V)
Taxane

CYP2C8(3*1)

CYP2C8(3*2)
Tamoxifen

CYP2D6*10(c.100C＞T)
Fluorouracil

DPYD(IVS14+1G>A)

MTHFR(C677T)
Irinotecan

UGT1A1(*6)
Cyclophosphamide

CYP2C9(c.430C＞T)

CYP2C9(c.1075A＞C)
Folate

MTHFR(c.1298A＞C)

MTHFR(c.677C＞T)

MTRR(c.66A＞G)
Nitroglycerin

ALDH2(c.1510G＞A)
Apoliporotein

APOE2(c.526C＞T)

APOE4(c.388T＞C)
Warfarin

VKORC1(c.-1639G＞A)

CYP2C9(c.1075A＞C)

CYP2C9(c.430C＞T)

CYP4F2(c.1165G＞A)
Clopidogrel

CYP2C19*2(c.681G＞A)

CYP2C19*3(c.636G＞A)

CYP2C19*17(c.-806C＞T)
Statin

SLCO1B1(c.521T＞C)

SLCO1B1(c.388A＞G)

APOE

APOE(c.388T＞C)
Diureticum

NPPA(T2238C)
Aspirin

LTC4S(c.-444A＞C)

GP1BA(c.482C＞T)

ITGB3(c.176T＞C)

PEAR1(c.-9-3996G＞A)

PTGS1(c.-842A＞C)
Calcium Channel Blockers（CCB）

CYP3A5*3(A6986G)
β-blockers

CYP2C6*10(c.100C＞T)

ADRB1(c.1165G＞A)
Allopurinol

HLA-B*5801
Angiotensin Converting 
Enzyme Inhibitors (ACEI)

ACE(I/D)
Angiotonin Receptor Blocker (ARB)

CYP2C9*3(c.1075A＞C)

AGTR1(c.1166A＞C)
Azathioprine
Mercaptopurine
Thioguanine

NUDT15(C415T)

TPMT(G238C)

TPMT(G460A)

TPMT(A719G)

AJ-Q9600 Multi-Channel Fluorescence Quantitative Analyzer

 

 

Basic performance Describe Basic performance Describe
Dimensions 410*320*196(mm) Weight 11kg
Power supply 220V Indicator light Standby breathing light, yellow-green switching indicator for heating and cooling
System performance Describe System performance Describe
Sample capacity 96 holes*0.2mL Sample volume 15-100uL
Applicable consumables 0.2mL single tube, 0.2mL*8 row tubes, 96-well plate Temperature control range 4-99℃
Hot lid temperature 30-108℃ (default 105℃, heated lid temperature is adjustable) Temperature control method High-performance imported semiconductors
Gradient temperature control 6-module gradient temperature control, maximum temperature difference supports 25°C Temperature control accuracy ±0.1℃
Temperature uniformity ±0.25° Temperature accuracy ±0.1℃
Operation function Preset projects and analysis parameters, simple and convenient operation Test quantity Detect 4 detection channels simultaneously
HRM detection Support Report Support report customization module, 96-well project printing
TouchDown function Support time and temperature increment and decrement mode Data Format XLS CSV DOCX

 

Fluorescence detection system performance Describe Fluorescence detection system performance Describe
Detection sensitivity Detects single copy genes Dynamic Range 1-10Λ10
Repeatability CV<1% Correlation coefficient 0.99-1
Detector High sensitivity photoelectric sensor Excite and detect propagation media High temperature resistant professional light guide fiber
Detection light wavelength 460nm-480nm， 525nm-545nm， 575nm-595nm， 600nm-620nm
Excitation light wavelength 505nm-525nm， 555nm-575nm， 600nm-620nm， 660nm-680nmv

Application scenario

1. Gene expression
2. Water body detection
3. Pathogen detection
4. Adulteration of meat products
5. Basic scientific research
6. Drug development and rational use of drugs
7. GMO testing and food safety testing

None

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